Detoxification of the phytoalexin brassinin by isolates of Leptosphaeria maculans pathogenic on brown mustard involves an inducible hydrolase.
Identifieur interne : 002274 ( Main/Exploration ); précédent : 002273; suivant : 002275Detoxification of the phytoalexin brassinin by isolates of Leptosphaeria maculans pathogenic on brown mustard involves an inducible hydrolase.
Auteurs : M Soledade C. Pedras [Canada] ; Ravi S. Gadagi ; Mukund Jha ; Vijay K. Sarma-MamillapalleSource :
- Phytochemistry [ 0031-9422 ] ; 2007.
Descripteurs français
- KwdFr :
- Ascomycota (enzymologie), Ascomycota (isolement et purification), Ascomycota (métabolisme), Biotransformation (MeSH), Cinétique (MeSH), Hydrolases (métabolisme), Hydrolases (physiologie), Indoles (composition chimique), Indoles (métabolisme), Moutarde (plante) (microbiologie), Protéines fongiques (métabolisme), Protéines fongiques (physiologie), Sesquiterpènes (MeSH), Spécificité du substrat (MeSH), Terpènes (composition chimique), Terpènes (métabolisme), Thiocarbamates (composition chimique), Thiocarbamates (métabolisme).
- MESH :
- composition chimique : Indoles, Terpènes, Thiocarbamates.
- enzymologie : Ascomycota.
- isolement et purification : Ascomycota.
- microbiologie : Moutarde (plante).
- métabolisme : Ascomycota, Hydrolases, Indoles, Protéines fongiques, Terpènes, Thiocarbamates.
- physiologie : Hydrolases, Protéines fongiques.
- Biotransformation, Cinétique, Sesquiterpènes, Spécificité du substrat.
English descriptors
- KwdEn :
- Ascomycota (enzymology), Ascomycota (isolation & purification), Ascomycota (metabolism), Biotransformation (MeSH), Fungal Proteins (metabolism), Fungal Proteins (physiology), Hydrolases (metabolism), Hydrolases (physiology), Indoles (chemistry), Indoles (metabolism), Kinetics (MeSH), Mustard Plant (microbiology), Sesquiterpenes (MeSH), Substrate Specificity (MeSH), Terpenes (chemistry), Terpenes (metabolism), Thiocarbamates (chemistry), Thiocarbamates (metabolism).
- MESH :
- chemical , chemistry : Indoles, Terpenes, Thiocarbamates.
- chemical , metabolism : Fungal Proteins, Hydrolases, Indoles, Terpenes, Thiocarbamates.
- enzymology : Ascomycota.
- isolation & purification : Ascomycota.
- metabolism : Ascomycota.
- microbiology : Mustard Plant.
- chemical , physiology : Fungal Proteins, Hydrolases.
- Biotransformation, Kinetics, Sesquiterpenes, Substrate Specificity.
Abstract
Brassinin is a phytoalexin produced by plants from the family Brassicaceae that displays antifungal activity against a number of pathogens of Brassica species, including Leptosphaeria maculans (Desm.) Ces. et de Not. [asexual stage Phoma lingam (Tode ex Fr.) Desm.] and L. biglobosa. The interaction of a group of isolates of L. maculans virulent on brown mustard (Brassica juncea) with brassinin was investigated. The metabolic pathway for degradation of brassinin, the substrate selectivity of the putative detoxifying hydrolase, as well as the antifungal activity of metabolites and analogs of brassinin are reported. Brassinin hydrolase activity was detectable only in cell-free homogenates resulting from cultures induced with brassinin, N'-methylbrassinin, or camalexin. The phytoalexin camalexin was a substantially stronger inhibitor of these isolates than brassinin, causing complete growth inhibition at 0.5mM.
DOI: 10.1016/j.phytochem.2007.03.020
PubMed: 17467751
Affiliations:
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Le document en format XML
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Ces. et de Not. [asexual stage Phoma lingam (Tode ex Fr.) Desm.] and L. biglobosa. The interaction of a group of isolates of L. maculans virulent on brown mustard (Brassica juncea) with brassinin was investigated. The metabolic pathway for degradation of brassinin, the substrate selectivity of the putative detoxifying hydrolase, as well as the antifungal activity of metabolites and analogs of brassinin are reported. Brassinin hydrolase activity was detectable only in cell-free homogenates resulting from cultures induced with brassinin, N'-methylbrassinin, or camalexin. The phytoalexin camalexin was a substantially stronger inhibitor of these isolates than brassinin, causing complete growth inhibition at 0.5mM.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17467751</PMID><DateCompleted><Year>2007</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>68</Volume><Issue>11</Issue><PubDate><Year>2007</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Detoxification of the phytoalexin brassinin by isolates of Leptosphaeria maculans pathogenic on brown mustard involves an inducible hydrolase.</ArticleTitle><Pagination><MedlinePgn>1572-8</MedlinePgn></Pagination><Abstract><AbstractText>Brassinin is a phytoalexin produced by plants from the family Brassicaceae that displays antifungal activity against a number of pathogens of Brassica species, including Leptosphaeria maculans (Desm.) Ces. et de Not. [asexual stage Phoma lingam (Tode ex Fr.) Desm.] and L. biglobosa. The interaction of a group of isolates of L. maculans virulent on brown mustard (Brassica juncea) with brassinin was investigated. The metabolic pathway for degradation of brassinin, the substrate selectivity of the putative detoxifying hydrolase, as well as the antifungal activity of metabolites and analogs of brassinin are reported. Brassinin hydrolase activity was detectable only in cell-free homogenates resulting from cultures induced with brassinin, N'-methylbrassinin, or camalexin. The phytoalexin camalexin was a substantially stronger inhibitor of these isolates than brassinin, causing complete growth inhibition at 0.5mM.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pedras</LastName><ForeName>M Soledade C</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, Canada. s.pedras@usask.ca </Affiliation> </AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gadagi</LastName><ForeName>Ravi S</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Jha</LastName><ForeName>Mukund</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sarma-Mamillapalle</LastName><ForeName>Vijay K</ForeName><Initials>VK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>04</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007211">Indoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012717">Sesquiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013729">Terpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013859">Thiocarbamates</NameOfSubstance></Chemical><Chemical><RegistryNumber>105748-59-2</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006867" MajorTopicYN="N">Hydrolases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007211" MajorTopicYN="N">Indoles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009149" MajorTopicYN="N">Mustard Plant</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012717" MajorTopicYN="N">Sesquiterpenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013729" MajorTopicYN="N">Terpenes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013859" MajorTopicYN="N">Thiocarbamates</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>01</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>9</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17467751</ArticleId><ArticleId IdType="pii">S0031-9422(07)00177-X</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2007.03.020</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Gadagi, Ravi S" sort="Gadagi, Ravi S" uniqKey="Gadagi R" first="Ravi S" last="Gadagi">Ravi S. Gadagi</name><name sortKey="Jha, Mukund" sort="Jha, Mukund" uniqKey="Jha M" first="Mukund" last="Jha">Mukund Jha</name><name sortKey="Sarma Mamillapalle, Vijay K" sort="Sarma Mamillapalle, Vijay K" uniqKey="Sarma Mamillapalle V" first="Vijay K" last="Sarma-Mamillapalle">Vijay K. Sarma-Mamillapalle</name></noCountry><country name="Canada"><noRegion><name sortKey="Pedras, M Soledade C" sort="Pedras, M Soledade C" uniqKey="Pedras M" first="M Soledade C" last="Pedras">M Soledade C. Pedras</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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